EGF-r gene copy number changes in renal cell carcinoma detected by fluorescencein situ hybridization

1998 ◽  
Vol 184 (4) ◽  
pp. 424-429 ◽  
Author(s):  
Holger Moch ◽  
Guido Sauter ◽  
Thomas C. Gasser ◽  
Lukas Bubendorf ◽  
Jan Richter ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Fluorescencein Situ Hybridization ◽  
R Gene ◽  
Copy Number Changes

scholarly journals VEGFA amplification/increased gene copy number and VEGFA mRNA expression in renal cell carcinoma with TFEB gene alterations

2018 ◽  
Vol 32 (2) ◽  
pp. 258-268 ◽  
Author(s):  
Anna Caliò ◽  
Matteo Brunelli ◽  
Diego Segala ◽  
Serena Pedron ◽  
Claudio Doglioni ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Mrna Expression ◽  
Renal Cell ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Gene Alterations

The Utility of ERBB4 and RB1 Immunohistochemistry in Distinguishing Chromophobe Renal Cell Carcinoma From Renal Oncocytoma

2019 ◽  
Vol 28 (3) ◽  
pp. 259-264
Author(s):  
Tong Sun ◽  
Lloyd Hutchinson ◽  
Amy G. Zhou ◽  
Qingqing Liu ◽  
Ediz F. Cosar ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Copy Number ◽  
Gene Copy Number ◽  
Chromophobe Renal Cell Carcinoma ◽  
Gene Copy ◽  
Renal Oncocytoma ◽  
Diagnostic Biomarker ◽  
Erbb4 Gene

Objectives. Differentiating renal oncocytoma (RO) from chromophobe renal cell carcinoma (ChRCC) can occasionally be challenging. We evaluated the expression of RB1 and ERBB4 in RO and ChRCC, and compared the immunohistochemistry (IHC) results to RB1 and ERBB4 gene abnormalities detected by fluorescence in situ hybridization (FISH). Materials and Methods. Fifty-three kidney resections (ChRCC, n=28; RO, n=25) were stained for RB1 and ERBB4 IHC and FISH was performed to evaluate gene copy number analysis. Results. A loss of RB1 staining was identified in 64% (18/28) of ChRCCs, which was not found in any ROs (0/25; P <.001). FISH analysis revealed 36% (10/28) of ChRCCs contained a RB1 hemizygous deletion with a concordance of 56% (10/18) between the IHC and FISH findings. No RB1 gene copy number variations were detected in any of the ROs (0/25; P <.001) and retained expression of RB1 by IHC. ERBB4 showed cytoplasmic/membranous staining in all ROs and ChRCCs. However, 75% (21/28) of ChRCCs also contained nuclear positivity for ERBB4, which was uncommonly seen in ROs (3/25, 12%; P < .001). A hemizygous ERBB4 gene deletion was detected in 46% of ChRCCs (13/28), but none of the ROs (0/25; 0%). Loss of labeling by RB1 or nuclear staining for ERBB4 IHC identified 25 of 28 (89%) of ChRCCs. Conclusion. In summary, the loss of RB1 expression is a highly specific diagnostic biomarker in distinguishing ChRCC from RO. Nuclear ERBB4 expression also appears to be a sensitive diagnostic biomarker for ChRCC, albeit the mechanism is unknown.

The EGFR Protein Expression and the Gene Copy Number Changes in Renal Cell Carcinomas

2009 ◽  
Vol 43 (5) ◽  
pp. 413 ◽  
Author(s):  
Sangho Lee ◽  
Jungsuk An ◽  
Aeree Kim ◽  
Young-Sik Kim ◽  
Insun Kim ◽  
...  
Keyword(s):  
Protein Expression ◽  
Renal Cell ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Renal Cell Carcinomas ◽  
Copy Number Changes

Clinical Relevance of Gene Copy Number Variation in Metastatic Clear Cell Renal Cell Carcinoma

2018 ◽  
Vol 16 (4) ◽  
pp. e795-e805 ◽  
Author(s):  
François-Xavier Nouhaud ◽  
France Blanchard ◽  
Richard Sesboue ◽  
Jean-Michel Flaman ◽  
Jean-Christophe Sabourin ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Renal Cell ◽  
Clinical Relevance ◽  
Copy Number ◽  
Clear Cell ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Cell Renal Cell Carcinoma ◽  
Gene Copy Number Variation ◽  
Number Variation

Array rank order regression analysis for the detection of gene copy-number changes in human cancer

Genomics ◽  
2003 ◽  
Vol 82 (2) ◽  
pp. 122-129 ◽  
Author(s):  
Chun Cheng ◽  
Robert Kimmel ◽  
Paul Neiman ◽  
Lue Ping Zhao
Keyword(s):  
Regression Analysis ◽  
Copy Number ◽  
Human Cancer ◽  
Rank Order ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Copy Number Changes
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